NEON Biorepository Carabid Collection (DNA Extracts) (NEON-CARC-DNA)

This collection contains genetic extracts derived from select ground beetle vouchers (Coleoptera: Carabidae; NEON sample class: bet_expertTaxonomistID_in.geneticSampleID). Ground beetles are sampled using pitfall traps (16 oz deli containers filled with 150 or 250 mL of diluted propylene glycol preservative). Multiple traps are deployed in each of 10 plots at each terrestrial NEON site (up to 4 traps per plot; maximum 40 traps per site), with traps arrayed approximately 20 meters from the center of the plot in each of the four cardinal directions. Sampling occurs biweekly throughout the growing season (when temperatures are above 4 degrees C). Following trap collection, all beetles from the family Carabidae are sorted by NEON technicians and identified to species or morphospecies. Annually, up to 95 Carabidae per site are pointed or pinned, receive secondary morphology-based identification by an expert taxonomist, and have genetic sequences produced at the cytochrome oxidase I barcode region. Beetles that are rare, particularly difficult to identify, or poorly represented in previous collection events are prioritized for DNA sequencing. Only beetle specimens that have been identified by an expert taxonomist are eligible for DNA barcoding. Barcodes of cytochrome oxidase I are generated per specimen and are cross-posted on the Barcode of Life Data Portal (http://www.boldsystems.org/). Genetic extracts in 96-well plates from the Canadian Centre for DNA Barcoding are archived in the NEON Biorepository at -80 degrees Celsius. See related links below for protocols and NEON related data products. Information about the Canadian Centre for DNA Barcoding can be found at https://ccdb.ca/

Contact: NEON Biorepository (biorepo@asu.edu)

Home Page: https://biorepo.neonscience.org/portal/collections/misc/collprofiles.php?collid=63

Collection Type: Preserved Specimens

Management: Data snapshot of local collection database

Digital Metadata: EML File

Usage Rights: CC0 1.0 (Public-domain)

• 9,165 occurrence
• 9,165 (100%) georeferenced
• 1 (0.01%) with images
• 7,945 (87%) identified to species
• 2 families
• 106 genera
• 527 species
• 535 total taxa (including subsp. and var.)